Plant Regeneration and Callus Formation from Chrysanthemum moriforium, C. coronarium and Lactuca sativa protoplasts
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چکیده
منابع مشابه
Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid
Embryogenic calli were induced from longitudinally bisected segments of protocorm-like bodies (PLBs) of Cymbidium Twilight Moon ‘Day Light’, a hybrid orchid, on modified Vacin and Went medium [Bot. Gaz. 110 (1949) 605] supplemented with 1-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) within 1 mont...
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In vitro propagation of C. morifolium cv. ‘Homa’ and cv. ‘Delkash’ and wild C. coccineum via direct and indirect organogenesis and somatic embryogenesis were investigated. BAP at 0, 1, 2 and 3 mg l-1 or NAA at 0, 0.05, 0.1 and 0.2 mg l-1 concentrations were used to induce direct and indirect organogenesis of shoot tip explants. To study the callu...
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To elucidate the chemical compositions of the aerial parts of Chrysanthemum coronarium L., the ethanol extracts of Ch. coronarium L. were firstly isolated by the MCI-gel resin column. The caffeoylquinic acid-rich fractions were further purified by various chromatographic columns including silica gel, Sephadex LH-20, and semi-preparative HPLC to yield the compounds. The purified compounds were c...
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The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...
متن کاملplant regeneration from protoplasts of lilium ledebourii (baker) boiss
for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...
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ژورنال
عنوان ژورنال: Journal of the Japanese Society for Horticultural Science
سال: 1989
ISSN: 0013-7626,1880-358X
DOI: 10.2503/jjshs.57.620